OrangerV1, Main, Exploration, bibRecord, 002725

Electrochemical protoplast fusion in citrus.

Identifieur interne : 002725 ( Main/Exploration ); précédent : 002724; suivant : 002726

Electrochemical protoplast fusion in citrus.

Auteurs : O. Olivares-Fuster [Espagne] ; N. Duran-Vila ; L. Navarro

Source :

Plant cell reports [ 0721-7714 ] ; 2005.

RBID : pubmed:15703946

English descriptors

KwdEn :
- Agriculture (instrumentation), Agriculture (methods), Cell Fusion (methods), Cell Proliferation (drug effects), Citrus sinensis (embryology), Citrus sinensis (genetics), Citrus sinensis (growth & development), Electric Stimulation, Electrochemistry, Hybrid Cells (drug effects), Hybrid Cells (metabolism), Membrane Fusion (drug effects), Membrane Fusion (physiology), Polyethylene Glycols (pharmacology), Protoplasts (drug effects), Protoplasts (physiology), Seeds (drug effects), Seeds (genetics), Seeds (growth & development).
MESH :
- chemical , pharmacology : Polyethylene Glycols.
- drug effects : Cell Proliferation, Hybrid Cells, Membrane Fusion, Protoplasts, Seeds.
- embryology : Citrus sinensis.
- genetics : Citrus sinensis, Seeds.
- growth & development : Citrus sinensis, Seeds.
- instrumentation : Agriculture.
- metabolism : Hybrid Cells.
- methods : Agriculture, Cell Fusion.
- physiology : Membrane Fusion, Protoplasts.
- Electric Stimulation, Electrochemistry.

Abstract

We report here the development of a novel protoplast fusion method for citrus somatic hybridization. This new procedure, which we have named electrochemical protoplast fusion, is based on chemically induced protoplast aggregation, using a low concentration of polyethylene glycol, and DC pulse-promoted membrane fusion. Based on the results of nucleus and mitochondria molecular analyses, we were successful in using this method to regenerate both symmetric somatic hybrids and cybrids. Various parameters, including pulse intensity, pulse length, and composition of the fusion media, were tested, and the optimum fusion condition selected consisted of two 100-micros pulses of 1,500 V cm(-1). Our conclusion is that electrochemical fusion is a reliable and reproducible method that combines the best features of both the chemical and electrical methods, thereby promoting cell division and high embryogenesis rates of the fused cells. It represents a new approach to citrus somatic hybridization. Various interesting features of this new approach are presented and discussed.

DOI: 10.1007/s00299-005-0916-1
PubMed: 15703946

Affiliations:

Espagne

Le document en format XML

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<front><div type="abstract" xml:lang="en">We report here the development of a novel protoplast fusion method for citrus somatic hybridization. This new procedure, which we have named electrochemical protoplast fusion, is based on chemically induced protoplast aggregation, using a low concentration of polyethylene glycol, and DC pulse-promoted membrane fusion. Based on the results of nucleus and mitochondria molecular analyses, we were successful in using this method to regenerate both symmetric somatic hybrids and cybrids. Various parameters, including pulse intensity, pulse length, and composition of the fusion media, were tested, and the optimum fusion condition selected consisted of two 100-micros pulses of 1,500 V cm(-1). Our conclusion is that electrochemical fusion is a reliable and reproducible method that combines the best features of both the chemical and electrical methods, thereby promoting cell division and high embryogenesis rates of the fused cells. It represents a new approach to citrus somatic hybridization. Various interesting features of this new approach are presented and discussed.</div>
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Electrochemical protoplast fusion in citrus.

Electrochemical protoplast fusion in citrus.

Source :

English descriptors

Abstract

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Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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